PRINCIPAL INVESTIGATORS (PI)

Dr Markus Rehm Lecturer, Research Lecturer Physiology & Medical Physics, RCSI, York House - York Street Dublin 2 mrehm@rcsi.ie
Dr. Markus Rehm leads an independent research group at the Royal College of Surgeons since 2005. He is appointed as Research Lecturer in Physiology and Biophysics. His Experimental Systems Biology group is part of the Department of Physiology and Medical Physics. Currently, the group of Dr. Rehm consists of 4 PhD students and 2 postdoctoral researchers covering expertise in Biochemistry, Biophysics, Molecular Imaging, and Computational Modeling. Dr. Rehm's group has established and utilized quantitative biochemistry and confocal and FRET-based imaging systems in combination with cell biological methods to monitor key processes during apoptosis execution in human cancer cells. This research in combination with complex computational systems analyses provides functional insight into the mechanisms of tumor resistance.
Research Interests	Research Projects	Research Team	Publications	Collaborations	Honours and Awards	CV

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CONTACT DETAILS

Lecturer, Research Lecturer
Physiology & Medical Physics, RCSI
York House - York Street, Dublin 2
mrehm@rcsi.ie
Telephone: 4028563
Mobile:
Fax: 4028639
Department Assistant: Patsy Connolly
Email: pconnolly2@rcsi.ie
Telephone:

EDUCATION
2003:	PhD, University of Osnabrueck, Germany. Cell Biology and Biophysics
1999:	MSc, University of Osnabrueck, Germany. Biophysics
1998:	BSc, University of Osnabrueck, Germany. Biology

RESEARCH AREAS

MEMBERSHIPS

International Cell Death Society (2007)

European Cell Death Organisation (2007)

Microscopy Society of Ireland (2005)

PERSONAL LINKS TO WEBSITES

http://systemsbiology.rcsi.ie

ORAL PRESENTATIONS

Rehm M Predictive live cell imaging elucidates the real-time kinetics of pore formation in the outer mitochondrial membrane and detects spatial waves of cytochrome-c release during apoptosis EWCD 2008, European Workshop on Cell Death, Hauenstein, Germany (Jun 2008)

Rehm M Quantitative real time analyses of the intracellular signaling dynamics during apoptosis execution. 15th ECDO Euroconference on Apoptosis, Portoroz, Slovenia (Oct 2007)

Rehm M Effector caspase activation as an all-or-none response and its control by X-linked inhibitor of apoptosis protein (XIAP) European Workshop on Cell Death, Rolduc, Netherlands (May 2006)

INVITED LECTURES

Rehm M tba Conway Lecture and Seminar Series, University College Dublin (Nov 2010)

Rehm M Imaging subcellular events during cell death The 18th Euroconference on Apoptosis & 7th Training course on Concepts and Methods in Programmed Cell Death, Ghent, Belgium (Sep 2010)

Rehm M FRET reporters in systems biology of apoptosis Nordic Imaging Network Workshop, University of Eastern Finland, Kuopio, Finland (May 2010)

Rehm M Killing cancer cells: Suicide signalling and death decisions Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) Seminar Series, Barcelona, Spain (Apr 2010)

Rehm M Expanding the Explanatory Power of Time Lapse Imaging by Mathematical Modelling of Biochemical Reaction Networks Zeiss - New Dimensions in Microscopy Workshop, Dublin, Ireland (Mar 2010)

Rehm M Real Time Analysis of Initiation and Execution of Apoptotic Cell Death by Single-Cell Imaging Biomedical Research Institute Seminar Series, University of Dundee, Scotland, UK (Jan 2010)

Rehm M Real Time Analysis of Initiation and Execution of Apoptotic Cell Death by Single-Cell Imaging Belgian Society of Microscopy Annual Conference, Gent, Belgium (Nov 2009)

Rehm M Choice cuts of death - Newly emerging pictures of cellular suicide Workshop on Cell death imaging, VIB - Ghent University, Belgium (Nov 2009)

Rehm M Biophotonic and systems biological investigation of cell death signaling Biochemistry Seminar Series, NUI Galway, Ireland (Oct 2009)

Rehm M Systems insights into apoptosis initiation Systems Biology Symposium, SBCancer/Bioquant, Heidelberg, Germany (May 2009)

Rehm M Integration of Extrinsic Cell Death Signals in Time and Space Molecular Medicine Seminar Series, University Medical Center Groningen, Netherlands (Mar 2009)

Rehm M Cellular death decision thresholds during apoptosis initiation and execution Irish Association for Cancer Research, Annual Meeting, Athlone, Ireland (Mar 2009)

Rehm M Combining quantitative Biochemistry, Microscopy and Systems Modeling to understand Apoptotic Signaling SBMC 2008 - Systems Biology of Mammalian Cells, Dresden, Germany (May 2008)

Rehm M Spatiotemporal analysis of caspase activation and mitochondrial permeabilisation during apoptosis ? novel imaging approaches Research Seminar Series, MRC Toxicology Unit, MRC Toxicology Unit, Leicester, UK (Feb 2008)

Rehm M Single cell analysis and computational modelling of apoptotic signalling Conference on Cellular and Molecular Biotechnology Applications in Medical Research, Santa Margherita Ligure, Italy (Feb 2008)

Rehm M Single cell analysis and computational modelling of apoptotic signalling IRTG Lecture series, Humboldt University, Berlin, Germany (Apr 2007)

Rehm M Combining live cell imaging and computational modeling EU FP6 COSBICS Winter School, Puerto de La Cruz, Tenerife, Spain (Mar 2007)

Rehm M Complex biological systems: Benefits from Software engineering Siemens PSE Life Science Symposium, Brno, Czech Republic (Mar 2006)

Rehm M Systems Biology in Single Cell Physiology SFI Workshop on Systems Biology, Hamilton Institute, Maynooth, Ireland (Jan 2005)

Rehm M A combination of Live Cell Microscopy and Computational Modelling identifies Key Players of Apoptotic Signalling Microscopic Society of Ireland, 29th annual symposium, DIT, Dublin, Ireland (Jan 2005)

Rehm M A Systems Biology approach to identify Key Players of Apoptotic Signalling Cell Biology & Immunology, University of Stuttgart, Germany (Jan 2005)

Rehm M A Systems Biology approach to identify Key Players of Apoptotic Signalling Center for Data Analysis and Modeling, University of Freiburg, Germany (Jan 2005)

Rehm M Real Time Single Cell Analysis of TNF-alpha-induced Apoptosis. DMMC Spring Symposium, Conway Institute of Biomolecular & Biomedical Research (Jan 2004)

Rehm M Single Cell Imaging DMMC Symposium, Trinity College Institute of Neuroscience & The Dublin Molecular Medicine Centre (Jan 2004)

PEER REVIEWED PUBLICATIONS

Würstle ML, Laussmann MA, Rehm M The Caspase-8 Dimerisation/Dissociation Balance is a highly potent Regulator of the Caspase-8, -3, -6 Signalling Loop J Biol Chem. 2010 Aug 11     PMID: 20702410      (Aug 2010)

Flanagan L, Sebastià J, Tuffy LP, Spring A, Lichawska A, Devocelle M, Prehn JH, Rehm M XIAP impairs Smac Release from the Mitochondria during Apoptosis Cell Death & Disease     PMID: 1, e49; doi:10.1038/cddis.2010.26      (Jun 2010)

Hellwig CT, Ludwig-Galezowska AH, Concannon CG, Litchfield DW, Prehn JH, Rehm M Activity of Protein Kinase CK2 uncouples Bid cleavage from Caspase-8 activation J Cell Sci. 2010 May 1;123(Pt 9):1401-6. Epub 2010 Mar 31     PMID: 20356928      (Mar 2010)

Huber HJ, Laussmann MA, Prehn JH, Rehm M Diffusion is capable of translating anisotropic Apoptosis Initiation into a homogeneous Execution of Cell Death BMC Syst Biol. 2010 Feb 4;4(1):9. [Epub ahead of print]     PMID: 20132548      (Jan 2010)

Wenus J, Dussmann H, Paul P, Kalamatianos D, Rehm M, Wellstead P, Prehn JH, Huber HJ ALISSA - An Automated Live-Cell Imaging System for Signal-Transduction Analyses Biotechniques. 2009 Dec;47(6):1033-40     PMID: 20041856      (Dec 2009)

Dussmann H, Rehm M, Concannon CG, Anguissola S, Wurstle M, Kacmar S, Voller P, Huber HJ, Prehn JH Single-Cell Quantification of Bax Activation and Mathematical Modeling Suggest Pore Formation upon Minimal Mitochondrial Bax Accumulation Cell Death Differ. 2009, Sep 11. [Epub ahead of print]     PMID: 19745831      (Sep 2009)

Anguissola S, Köhler B, O?Byrne R, Düssmann H, Rehm M, Kögel D, Prehn JH Bid and Calpains cooperate to trigger Oxaliplatin-induced Apoptosis of Cervical Carcinoma HeLa Cells Mol Pharmacol. 2009 Aug 27. [Epub ahead of print]     PMID: 19713356      (Aug 2009)

Rehm M, Huber HJ, Hellwig CT, Anguissola S, Dussmann H, Prehn JH Dynamics of Outer Mitochondrial Membrane Permeabilization during Apoptosis Cell Death Differ. 2009 Apr;16(4):613-23. Epub 2009 Jan 9.     PMID: 19136937      (Apr 2009)

Farrelly AM, Wobser H, Bonner C, Anguissola S, Rehm M, Concannon CG, Prehn JH, Byrne MM Early loss of mammalian target of rapamycin complex 1 (mTORC1) signalling and reduction in cell size during dominant-negative suppression of hepatic nuclear factor 1-alpha (HNF1A) function in INS-1 insulinoma cells Diabetologia. 2009 Jan;52(1):136-44. Epub 2008 Oct 24     PMID: 18949455      (Oct 2008)

O'Connor CL, Anguissola S, Huber HJ, Dussmann H, Prehn JH, Rehm M Intracellular signaling dynamics during apoptosis execution in presence or absence of X-linked-inhibitor-of-apoptosis-protein Biochim Biophys Acta. 2008 Oct;1783(10):1903-13     PMID: 18590777      (Oct 2008)

Rudy A, López-Antón N, Barth N, Pettit GR, Dirsch VM, Schulze-Osthoff K, Rehm M, Prehn JH, Vogler M, Fulda S, Vollmar AM Cephalostatin-mediated apoptosis is Smac-dependent and involves assembly of the PIDDosome. Cell Death Differ. 2008 Dec;15(12):1930-40. Epub 2008 Sep 19.     PMID: 18806754      (Sep 2008)

Huber HJ, Plchut M, Weisova P, Dussmann H, Rehm M, Ward MW, Prehn JH TOXI-SIM ? A simulation tool for the analysis of mitochondrial and plasma membrane potentials J Neurosci Methods. 2009 Jan 30;176(2):270-5. Epub 2008 Sep 7.     PMID: 18824028      (Sep 2008)

Köhler B, Anguissola S, Concannon CG, Rehm M, Kögel D, Prehn JH Bid participates in genotoxic drug-induced apoptosis of cervical cancer HeLa cells and is essential for the apoptotic and synergistic effects of death receptor ligands PLoS One. 2008 Jul 30;3(7):e2844.     PMID: 18665234      (Aug 2008)

Hellwig CT, Kohler BF, Lehtivarjo A, Dussmann H, Courtney MJ, Prehn JH, Rehm M Real-time analysis of TRAIL/CHX-induced caspase activities during apoptosis initiation J Biol Chem. 2008 Aug 1;283(31):21676-85. Epub 2008 Jun 3.     PMID: 18522940      (Jun 2008)

Konig HG, Rehm M, Gudorf D, Krajewski S, Gross A, Ward MW, Prehn JH Full length Bid is sufficient to induce apoptosis of cultured rat hippocampal neurons. BMC Cell Biol. 2007 Feb 27;8:7.     PMID: 17326836      (Feb 2007)

Huber HJ, Rehm M, Plchut M, Dussmann H, Prehn JH APOPTO-CELL - A simulation tool and interactive database for analyzing cellular susceptibility to apoptosis Bioinformatics. 2007 Mar 1;23(5):648-50. Epub 2007 Jan 19     PMID: 17237051      (Jan 2007)

Rehm M, Huber H, Dussmann H, Prehn JH Systems analysis of effector caspase activation and its control by X-linked inhibitor of apoptosis protein EMBO J. 2006 Sep 20;25(18):4338-49     PMID: 16932741      (Sep 2006)

Ward MW, Rehm M, Duessmann H, Kacmar S, Concannon CG, Prehn JH Real time single cell analysis of BID cleavage and BID translocation during caspase-dependent and neuronal caspase-independent apoptosis J Biol Chem;281(9):5837-44     PMID: 16407197      (Dec 2005)

Rehm M, Dussmann H, Prehn JH Real-time single cell analysis of Smac/DIABLO release during apoptosis J Cell Biol 162(6):1031-43     PMID: 12975347      (Sep 2003)

Dussmann H, Kogel D, Rehm M, Prehn JH Mitochondrial membrane permeabilization and superoxide production during apoptosis. A single-cell analysis J Biol Chem 278(15):12645-9     PMID: 12560329      (Apr 2003)

Dussmann H, Rehm M, Kogel D, Prehn JH Outer mitochondrial membrane permeabilization during apoptosis triggers caspase-independent mitochondrial and caspase-dependent plasma membrane potential depolarization: a single-cell analysis J Cell Sci 116(Pt 3):525-36     PMID: 12508113      (Feb 2003)

Rehm M, Dussmann H, Janicke RU, Tavare JM, Kogel D, Prehn JH Single-cell fluorescence resonance energy transfer analysis demonstrates that caspase activation during apoptosis is a rapid process. Role of caspase-3 J Biol Chem 277(27):24506-14     PMID: 11964393      (Jul 2002)

BOOK CHAPTERS

Huber H, Bullinger E, Rehm M Systems approaches to the study of apoptosis In: Essentials of Apoptosis: A Guide for Basic and Clinical Research (Ed. X.M. Yin, Z.Dong), Humana Press, NJ, USA. in print      (Mar 2008)

Huber H, Gomez Estrada G, Dussmann H, O'Connor C, Rehm M Extending the Explanatory Power of Live Cell Imaging by com-putationally modelling the Execution of Apoptotic Cell Death In: Modern Research and Educational Topics in Microscopy, Vol. 1; Formatex Microscopy Book Series, Formatex, 2007. ISBN-13: 978-84-611-9419-3 - http://www.formatex.org/microscopy3/pdf/pp88-99.pdf      (Oct 2007)

THESES

Student Name: Eugenia Degado Martin (PhD / 4 yrs) Thesis Title: Awarding Institution:

Student Name: Maximilian Wuerstle (MRes/PhD / 4 yrs) Thesis Title: Systems Analysis of Apoptotic Cell Death Processes Awarding Institution:

Student Name: Maike Laussmann (PhD) Thesis Title: A qualitative and quantitative evaluation of of novel caspase activating compounds. Awarding Institution:

Student Name: Lorna Flanagan (PhD) Thesis Title: Real time detection of cell death signaling at the Interface between Apotosis Initiation & Execution. Awarding Institution:

Student Name: Christian Hellwig (PhD) Thesis Title: Real-Time Analyses of Intracellular Apoptotic Signalling Networks Awarding Institution:

TRAINING INITIATIVES

Lecturer in MRes/PhD Programme Lecturer  (2004)

Lecturer, Integrated Tutorials in Biochemistry and Physiology, School of Pharmacy Lecturer  (2006)

School on Systems Biology for Medical Applications Supported by the European Commission (FP6 projects COSBICS and SysBioMed) Lecturer  (2006)

Physics of the Cardiovascular and Respiratory System Lecturer  (2007)

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