Royal College of Surgeons in Ireland Coláiste Ríoga na Máinleá in Éirinn

Dr Carolina Garciarena

Carolina Garciarena

Dr. Carolina Garciarena, PhD
Honorary Lecturer
Email: carolinagarciarena@rcsi.com  

Biography
Carolina Garciarena graduated in Biochemistry and subsequently received a PhD in Cardiovascular Physiology, mentored by Prof Ennis and Prof. Cingolani, at the University of La Plata, Argentina. In 2009, she joined Prof. Vaughan-Jones group at the University of Oxford as a British Heart Foundation postdoctoral researcher, to explore the role of H+ and Ca2+ in cardiac pathophysiology applying microfluidic techniques and confocal imaging. In 2015 she moved to Ireland to join the Cardiovascular Infection group led by Dr. Steve Kerrigan at RCSI. Her current work focuses on the study endothelial dysregulation following bacterial infection using in vitro 3D microvessels.

Research interests
Bloodstream infection-sepsis
Vascular pathophysiology
Dynamic bacteria-host interactions
3D models of disease

Publications
23. Inhibition of major integrin aVb3 reduces Staphylococcus aureus attachment to sheared human endothelial cells. McDonnell CJ, Garciarena CD, Watkin RL, McHale TM, McLoughlin A, Claes J, Verhamme P, Cummins PM, Kerrigan SW. J Thromb Haemost. 2016 Sep 8. doi: 10.1111/jth.13501.In press.

22. Towards 3D in vitro models for the study of cardiovascular tissues and disease. Ryan AJ, Brougham CM, Garciarena CD, Kerrigan SW, O'Brien FJ. Drug Discov Today. 2016 Sep;21(9):1437-45.

21. Coordinated Molecular Cross-Talk between Staphylococcus aureus, Endothelial Cells and Platelets in Bloodstream Infection. Garciarena CD, McHale T, Watkin R, Kerrigan SW. Pathogens. 2015 Dec 5;4(4):869-82

20. H+-activated Na+ influx in the ventricular myocyte couples Ca2+-signalling to intracellular pH. Garciarena CD, Youm JB, Swietach P, Vaughan-Jones RD.
Journal of Molecular and Cellular Cardiology. Volume 61, August 2013, Pages 51-59.

19. Sarcolemmal localisation of Na+/H+ exchange and Na+-HCO3- co-transport influences the spatial regulation of intracellular pH in rat ventricular myocytes. Garciarena CD, Ma YL, Swietach P, Huc L, Vaughan-Jones RD. May 1, 2013 The Journal of Physiology, 591, 2287-2306. Recommended by F1000Prime:
http://f1000.com/prime/718003607?subscriptioncode=2e6331b9-d77c-4c66-a112-5845a13db2ec

18. Myocardial reperfusion injury: Reactive oxygen species vs. NHE-1 reactivation. Garciarena CD, Fantinelli JC, Caldiz CI, Chiappe de Cingolani G, Ennis IL, Pérez NG, Cingolani HE, Mosca SM. Cell Physiol Biochem. 2011;27(1):13-22.

17. Phosphodiesterase 5A inhibition decreases NHE-1 activity without altering steady state pHi: Role of phosphatases. Díaz RG, Nolly MB, Massarutti C, Casarini MJ, Garciarena CD, Ennis IL, Cingolani HE, Pérez NG. Cell Physiol Biochem. 2010;26(4-5):531-40.

16. Decreased Activity of the Na+/H+ Exchanger by Phosphodiesterase 5A Inhibition Is Attributed to an Increase in Protein Phosphatase Activity. Yeves AM, Garciarena CD, Nolly MB, Chiappe de Cingolani GE, Cingolani HE, Ennis IL. Hypertension. 2010 Oct;56(4):690-5.

15. Role of reactive oxygen species (ROS) in angiotensin II-induced stimulation of the cardiac Na+/HCO3- cotransport. De Giusti VC, Garciarena CD, Aiello EA. J Mol Cell Cardiol. 2009;47(5):716-22.

14. Endurance Training in the Spontaneously Hypertensive Rat. Conversion of Pathological into Physiological Cardiac Hypertrophy. Carolina D. Garciarena, Oscar A. Pinilla, Mariela B. Nolly, Ruben P. Laguens, Eduardo M. Escudero, Horacio E. Cingolani, and Irene L. Ennis. Hypertension. 2009 Apr;53(4):708-14.

13. Chronic NHE-1 blockade induces an antiapoptotic effect in the hypertrophied heart. Garciarena CD, Caldiz CI, Portiansky EL, Chiappe de Cingolani GE, Ennis IL. J Appl Physiol. 2009 Apr;106(4):1325-31.

12. Na+/H+ exchanger-1 inhibitors decrease myocardial superoxide production via direct mitochondrial action. Garciarena CD, Caldiz CI, Correa MV, Schinella GR, Mosca SM, Chiappe de Cingolani GE, Cingolani HE, Ennis IL. J Appl Physiol, Dec 2008; 105: 1706-13.

11. Early signals after stretch leading to cardiac hypertrophy. Key role of NHE-1. Cingolani HE, Pérez NG, Aiello EA, Ennis IL, Garciarena CD, Villa-Abrille MC, Dulce RA, Caldiz CI, Yeves AM, Correa MV, Nolly MB, Chiappe de Cingolani G. Front Biosci. 2008 May 1;13:7096-114.

10. Is cardiac hypertrophy from Spontaneously Hypertensive Rats the cause or the consecuence of oxidative stress? Álvarez MC, Caldiz CI, Fantinelli JC, Garciarena CD, Console GM, Chiappe de Cingolani GE, Mosca SM. Hypertension Research, Vol. 31 (2008) No. 7 pp.1465-1476.

9. From Anrep's Phenomenon to myocardial hypertrophy: Role of the Na+/H+ exchanger. Ennis IL, Cingolani HE, Garciarena CD, Camilión de Hurtado MC, Villa-Abrille MC, Aiello EA and Pérez NG. Current Cardiology Reviews, 3:149-164, 2007.

8. Mitochondrial reactive oxygen species activate the slow force response to stretch in feline myocardium. Caldiz CI, Garciarena CD, Dulce RA, Novaretto LP, Yeves AM, Ennis IL, Cingolani HE, Chiappe de Cingolani G, Pérez NG. J Physiol. 2007 Nov 1;584(Pt 3):895-905.

7. Normalization of the calcineurin pathway underlies the regression of hypertensive hypertrophy induced by NHE-1 inhibition. Irene L. Ennis, Carolina D Garciarena, Eduardo M Escudero, Néstor G. Pérez, Raúl A. Dulce, María C Camilión de Hurtado and Horacio E. Cingolani. Can J Physiol Pharmacol. 2007;85(3-4):301-10.

6. Phosphodiesterase 5A inhibition induces Na+/H+ exchanger blockade and protection against myocardial infarction. Pérez NG, Piaggio MR, Ennis IL, Garciarena CD, Morales C, Escudero EM, Cingolani OH, Chiappe de Cingolani G, Yang XP, Cingolani HE. Hypertension. 2007 May;49(5):1095-103.

5. Echocardiographic analysis of the effect of different Na+/H+ exchanger inhibitors on left ventricular structure and function in spontaneously hypertensive rats. Escudero EM, Tufare AL, Ennis IL, Garciarena CD, Pinilla OA, Carranza VB. Medicina (B Aires). 2006;66(5):392-8.

4. Angiotensin II-induced endothelin-1 release in cardiac myocytes. Villa-Abrille MC, Cingolani HE, Garciarena CD, Ennis IL, Aiello EA. Medicina (B Aires). 2006;66(3):229-36.

3. Endothelin-1 induced hypertrophic effect in neonatal rat cardiomyocytes: Involvement of Na(+)/H(+) and Na(+)/Ca(2+) exchangers. Dulce RA, Hurtado C, Ennis IL, Garciarena CD, Alvarez MC, Caldiz C, Pierce GN, Portiansky EL, Chiappe de Cingolani GE, Camilion de Hurtado MC. J Mol Cell Cardiol. 2006 Nov;41(5):807-15.

2. The positive inotropic effect of angiotensin II: role of endothelin-1 and reactive oxygen species. HE Cingolani, MC Villa-Abrille, M Cornelli, A Nolly, IL Ennis, C Garciarena, AM Suburo, V Torbidoni, MV Correa, MC Camilión de Hurtado, EA Aiello.
Hypertension. 2006 Apr;47(4):727-34.

1. Endothelin isoforms and the response to myocardial stretch. Ennis IL, Garciarena CD, Perez NG, Dulce RA, Camilion de Hurtado MC, Cingolani HE. Am J Physiol Heart Circ Physiol. 2005 Jun;288(6):H2925-30.